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Wound healing assay Isolated PSCs were cultured in 6-well plates for 72 h
The conflicting findings regarding M1 and M2 macrophage polarization suggest that the effects of NAC may be context-dependent, influenced by factors such as concentration, cell type, and specific microenvironmental conditions
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For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method
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