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The column was pre-equilibrated with 10 column volumes (CV) of 1 Dulbeccos Phosphate Buffered Saline (DPBS) (pH 7.4) (Sigma-Aldrich)/0.05% (v/v) sodium azide (Sigma-Aldrich), before the supernatant was loaded onto the column at a flow rate of (0.52) mL/min
A simple formula guides solvent volume calculation
in last step, all restraints were remove to equilibrate for 2 ns
Bellenguez C, Kkali F, Jansen IE, Kleineidam L, Moreno-Grau S, Amin N, et al
Glutathione is created in our bodies with a combination of 3 amino acids, cysteine, glutamic acid, and glycine
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