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Mains et al., 2021)

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Hold the vial upright when drawing so the needle tip is submerged below any surface bubbles

Images were collected using a CFI Plan Apochromat Lambda D oil immersion objective (60, NA = 1.4) and for roGFP imaging a CFI Plan Apo VC water immersion objective (60, NA = 1.2)

Lentiviral transduction of mRFP-GFP-LC3 shRNA and autophagic flux determination Lentiviruses encoding short hairpin RNAs (shRNA) to human mRFP-GFP-LC3-puro were constructed by Hanbio Biotechnology (Hanbio, Shanghai, China)

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